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KMID : 0378019610040110055
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New Medical Journal
1961 Volume.4 No. 11 p.55 ~ p.67
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Studies on the Influences of Long Term Administration of Red Pepper Upon Rabbits
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Abstract
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The present study was undertaken to investigate the histo-pathological changes of various organs including liver, spleen, bone marrow, stomach, intestine, heart, lung, and kidney of rabbits fed with red peppers in long term(13 months). Twenty eight rabbits were divided into 4 groups(Group A,B,C, and D). Group A was fed only with normal standard diet, while group B,C and D were fed with 0.5g, 5g and 10g of red peppers; per kg . of body weight respectively in addition to normal standard diet. After 13 months feeding, the animals were sacrificed, and histopathological changes of the various organs were studied. Group A and B did not show any pathological changes, while Group C and D revealed various changes in all studied organs, the results were summarized as follows.
LIVER: Sections of the liver show multiloculated pseudolobules which are bounded by portal mesenchymal fibrous tissue with cellular proliferation, consisting of mainly lymphocytes, plasma cells, so-called´small round cell infiltration and lesser degrees or numbers of neutrophiles. In hepatic cells all throughout areas show moderate to marked degrees of extracellular and intracellular fatty infiltration, so called fatty metamorphosis and associated with parenchymatous degeneration and necrotic process, revealing that rare giant cell formation eosinophilic degeneration or bodies formation, cloudy and edematous swelling, pyknotic and _karryolytic, neuclei to almost disappeared hepatic cells . replacing by . collagenous eosinophilic connective tissue, circumscribed and multiple. The central veins are dilated and filled with. congestion :and developed_ central zone and peripheral zonal mild fibrosis and edematous changes, this changes indicating that are seem to be chronic passive congestion. Bile ducts in portal spaces show mild to moderate degrees of proliferation of the epithelium and contains mild amount of inspisated bile in some areas. In special Stains, such as Van Gieson´s for collagenous connective tissue in stromal area reveals interlacing fibrous bundles extending toward hepatic parenchyma. Glison´s capsule is moderately thickened by fibrous tissue. These of discribed as above mentioned; patho-histologic findings of the liver are observed particularly in Group C and D compared with control groups of A and B.
.SPLEEN: The splenic,capsule and trabecule show diffuse thickening by the hyalinized fibrous connective tissue. The red pulps are markedly dilated and filled with passive congestion all throughout areas. The endothelial cells of the pulp developed marked degrees of proliferation with thickened sinus wall by increased amounts of hyalinized fibro-elastic connective tissue, forming so-called pseudo-fibro-adeni. Scattered white pulps show marked degrees of atrophic changes and compression probably due to secondary changes of red pulpular passive congestion. Central arteries in white pulps developed marked degrees of haylinization.
BONE MARROW: H and E sections of the bone marrow show increased cellularity, 70% ´to 90% and marked degrees . of erythrocytic hyperplasia consisting mainly of erythroblasts and prominent numbers of normocytes by Giemsa´s staining. Myelocytic series also studied by H & E staining show , depressed ,and, decreased distribution, consisting of myeloblast, myelocytes and few numbers of mature neutrophiles. Lymphocytes and plasma cells are not observed by Giemsa´s staining. M:E, ratio reveals 1 to 6.
STOMACH: The mucosal membrane show increased cellularity indieating,that the proliferative changes. The basis of glands to the submucosal areas are infiltrated by small round cells infiltration consisting of mainly lymphocytes and lesser numbers of the plasma cells. The muscular coat shows proliferation, and increased fibrous connective tissue, forming ,thickened wall. Serosal layer is not. xo remarkable. The erosive changes to ulceration of the mucosa all throughout are not observed. Small intestine and large intestine show hyperplasia and thickened wall, otherwise are similar findings to describe as above findings in stomach.
HEART: Myocardium shows mild degrees of fragmentation and dissociation of the bundles and rare myocardial giant cells. The interstitial congestion are observed all throughout areas. Epicardium and endocardium including valvular structures are not. remarkable to describe.
LUNG: Lung parenchyme reveals compensatory focal atelectasis and emphysematous changes. Intgi;estink findings of the pulmonary vessels show artherosclerotic intimal and medial thickening, developing almost narrowing or toward to obliteration of the lumen. The artherosclerosis of the pulmonary vessels are seemed to be causing pulmonary hypertension particularly in these studied rabbits which has been suffered.
KIDNEY: The glommerulus in the cortex show congestive changes and edema. The Bowmann´s capsule and glommerular tufts are lined by endothelial layer and completely patented capsular spaces. Tubular changes all throughout the areas show parenchymatous degeneration such as cloudy swelling, and desquamation of the epithelium and containing hyaline casts in the lumens. Blood vessels are those of normal limit. The interstitium show edematous changes all throughout.
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